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three% of all one point mutations in DHFR are beneficial from the absence of Lon, but advantageous mutations are mainly suppressed when Lon is reintroduced. Protein steadiness measurements reveal intensive activity-balance tradeoffs for your advantageous mutants and supply a mechanistic rationalization for Lon’s common impact. Our results counsel opportunities for tuning mutational landscapes by modulating the mobile atmosphere, with implications for protein structure and combatting antibiotic resistance.
(2020) Altered expression of an outstanding Management protease in E. coli reshapes the in vivo mutational landscape of the design enzyme
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(2020) Altered expression of a high quality Management protease in E. coli reshapes the in vivo mutational landscape of a design enzyme